Hemoglobin binding activity and hemoglobin-binding protein of prevotella nigrescens

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Hemoglobin binding activity and hemoglobin-binding protein of Prevotella nigrescens

Prevotella nigrescens, lacking siderophores was found to bind to the hemoproteins. The binding was observed also in the envelope which was prepared by sonication of the cell. The binding occurred in the pH-dependent manner; the binding was observed below neutral pHs of the incubation mixtures but only slightly observed in the neutral and alkaline pHs. Furthermore, hemoglobin bound to the envelo...

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Prevotella intermedia and Prevotella nigrescens serotypes, ribotypes and binding characteristics.

Type strains and 62 clinical isolates of Prevotella intermedia and Prevotella nigrescens were typed with the use of genomic DNA fingerprints and rRNA gene probes. The strains were further serotyped with monoclonal antibodies and characterized with SDS-PAGE, enzymatic activities, hemolysis and hemagglutination and coaggregation with Streptococcus and Actinomyces spp. P. intermedia and P. nigresc...

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Binding and utilization of human transferrin by Prevotella nigrescens.

To survive and multiply within their hosts, pathogens must possess efficient iron-scavenging mechanisms. In the present study, we investigate the capacity of Prevotella nigrescens and Prevotella intermedia to use various sources of iron for growth and characterize the transferrin-binding activity of P. nigrescens. Iron-saturated human transferrin and lactoferrin, but not ferric chloride and the...

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Serum protein binding of myoglobin, hemoglobin and hematin.

ECENTLY much attention has been focused on the protein binding of hemoglobin. The hemoglobin binding proteins, collectively called haptoglobin, have been thoroughly studied by several workers.’5 Indentification of these substances has helped to clarify the concept of “renal threshold” for hemoglobin excretion.5’6’9 Subsequently, in an investigation of the difference in “renal threshold” of myog...

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ژورنال

عنوان ژورنال: European Journal of Medical Research

سال: 2010

ISSN: 2047-783X

DOI: 10.1186/2047-783x-15-7-314